Becton Dickinson (BD) LSRFortessa

This platform at CLNS-IIT@Sapienza provides basic support to the projects in the field of flow
cytometry, as well as help in the development of novel protocols and methodology. The laboratory
technology has the main purpose to characterize and isolate a variety of cell population both of human
and mouse origin.
Flow Cytometry platform setup
The flow cytometry laboratory is equipped with two laser-based instruments which technology allows
simultaneous multiparametric analysis of thousands of cells for second for rapid analysis and/or
isolation of complex cell populations. This is achieved by the use of different labeled antibodies or
dyes to target specific molecules express at the surface or in the cytoplasm of the cell, giving a
screening able to describe in detail a complex sample or to identify low frequency subset of cells.
Through these instruments, a variety of fluorochromes attached to different antibodies can screen the
molecular components of a cell first to characterize it (flow cytometer) and then to specifically select
for isolation (cell sorter). The following machinery have been purchased:
• The BD LSRFortessa cell analyzer is a 5 laser cytometer (488nm, UV 355nm, V 405nm,
561nm and 640nm) and 18 fluorescence detectors running a BD FACSDiva software on XP.
The main feature of the cell analyzer is to characterize a given cell population.
• The BD FACSAriaIII is a 4 laser cell sorter (488nm, Near UV 375nm, 561 nm and 640nm)
and 10 fluorescence detectors running a BD FACSDiva software on XP. The main feature of
the cell sorter is to isolate a specific population.