Neon Transfection System - ThermoFisher Scientific Instrument description Cell electroporator Description and functionality The Neon Transfection System offers an innovative electroporation transfection method that utilizes a proprietary biologically compatible pipette tip chamber to generate a more uniform electric field for a significant increase in transfection efficiency and cell viability. Up to 90% transfection and gene-editing efficiency in extremely difficult cells, including immune, primary and stem cells; over 140 cell lines were tested with optimized ready-to-use conditions, efficiency and viability. Open system allows electroporation parameters to be optimized freely; easily transfect from 2 x 10*4 cells to 6 x 10*6 cells per reaction. Can be used to deliver DNA, RNA and protein. Unlike standard cuvette-based electroporation chambers, the Neon system uses a proprietary biologically compatible pipette tip chamber that generates a more uniform electric field. This design allows a better maintenance of physiological conditions resulting in very high cell survival compared to conventional electroporation. Read more about Neon Transfection System - ThermoFisher Scientific Attachment Quick reference card Manual User bulletin: CRISPR/Cas9-mediated Genome Editing of Human Primary T-cells location and contact persons Contact person alessandro.rosa Experienced users alessandro.rosa Laboratory denomination IIT-CLNS wet lab Department or Institution IIT Building RM111 Floor 4 Room Lab 29 Sapienza institutional funding (Finanziamento di Ateneo) other external funding safety notes Special safety instructions The Neon® Transfection System including the Neon® Pipette Station is compatible with standard nonhazardous laboratory reagents. Do not use organic solvents in the tip/tubes or with the device. Always ensure that the power supply input voltage matches the voltage available in your location. For operating environment, see page 35 of the manual. This device is air-cooled so its surfaces become hot during operation. When installing the device, leave a space of more than 10 cm (4 inches) around it. Never insert metallic objects into the air vents of the device as this could result in electrical shock, personal injury and equipment damage. Always set the main switch on the power supply unit to OFF before connecting the power cord to the wall outlet. Always ensure that the grounding terminal of the device and that of the wall outlet are properly connected. Connect the power cord to a grounded, 3-conductor power outlet. To avoid potential shock hazard, make sure that the power cord is properly grounded. Be sure to position the instrument such that it is easy to disconnect the unit. Be sure to set the main switch to OFF, unplug the power cord, and secure the pipette station before moving the device. Safety data notes Potential shock hazard
